DNA damage in mussels at sites in San Diego Bay

作者: Scott A Steinert , Rebecca Streib-Montee , James M Leather , David B Chadwick

DOI: 10.1016/S0027-5107(97)00267-4

关键词: MusselBivalviaEcologyEnvironmental chemistryBayComet assayBiologyBioaccumulationMytilusContaminationWater pollution

摘要: Abstract Identification and assessment of introduced other toxicants is crucial to any comprehensive study contaminants within the marine environment. The relationship between DNA single-strand breaks exposure organisms environmental was examined at sites in San Diego Bay, CA. A extent consequences contamination area Naval Station conducted summer 1995. addressed sources, distributions, concentrations, transport, sediment–water exchange, biological effects, degradation. effects portion (this paper) included contaminant bioaccumulation, organismal growth, determination using Comet assay. damage determined hemocytes collected from deployed resident mussels, Mytilus edulis , six stations around Diego. Deployed mussels were exposed on station for ∼30 days plastic mesh bags, placed 1 m above bottom. Hemocyte samples 0, 12, 32. It found that exhibiting extremes exposure, both highest lowest easily identified growth measurements. Sediment chemistry bioaccumulation data indicated, Hg, Cu, Zn, be most notable contaminants. assay, particular germ cell determinations, respond rapidly Results this an earlier 1993 suggest non-sediment associated observed one may have been result photoactivation accumulated PAHs.

参考文章(31)
Xiao-Dong Huang, D. George Dixon, Bruce M. Greenberg, Impacts of UV radiation and photomodification on the toxicity of pahs to the higher plant Lemna gibba (duckweed Environmental Toxicology and Chemistry. ,vol. 12, pp. 1067- 1077 ,(1993) , 10.1002/ETC.5620120613
Narendra P Singh, Michael T McCoy, Raymond R Tice, Edward L Schneider, None, A simple technique for quantitation of low levels of DNA damage in individual cells Experimental Cell Research. ,vol. 175, pp. 184- 191 ,(1988) , 10.1016/0014-4827(88)90265-0
D.B. Chadwick, M.H. Salazar, Integrated Measurement Technologies For Monitoring The Marine Environment oceans conference. ,vol. 1, pp. 343- 350 ,(1991) , 10.1109/OCEANS.1991.613953
Scott R. Carr, Duane C. Chapman, Edward R. Long, Herbert L. Windom, Glen Thursby, Gail M. Sloane, Douglas A. Wolfe, Sediment quality assessment studies of Tampa bay, Florida Environmental Toxicology and Chemistry. ,vol. 15, pp. 1218- 1231 ,(1996) , 10.1002/ETC.5620150730
Edward R. Long, Donald D. Macdonald, Sherri L. Smith, Fred D. Calder, Incidence of adverse biological effects within ranges of chemical concentrations in marine and estuarine sediments Environmental Management. ,vol. 19, pp. 81- 97 ,(1995) , 10.1007/BF02472006
William R. Gala, John P. Giesy, Flow cytometric determination of the photoinduced toxicity of anthracene to the green alga Selenastrum Capricornutum Environmental Toxicology and Chemistry. ,vol. 13, pp. 831- 840 ,(1994) , 10.1002/ETC.5620130519
Carol Bernstein, Harris Bernstein, Chapter Two – DNA Damage Aging, Sex, and DNA Repair. pp. 15- 26 ,(1991) , 10.1016/B978-0-12-092860-6.50006-5
Björn Rydberg, Karl J. Johanson, ESTIMATION OF DNA STRAND BREAKS IN SINGLE MAMMALIAN CELLS DNA Repair Mechanisms. pp. 465- 468 ,(1978) , 10.1016/B978-0-12-322650-1.50090-4
J. P. Meador, J. E. Stein, W. L. Reichert, U. Varanasi, Bioaccumulation of Polycyclic Aromatic Hydrocarbons by Marine Organisms Reviews of Environmental Contamination and Toxicology. ,vol. 143, pp. 79- 165 ,(1995) , 10.1007/978-1-4612-2542-3_4
P. Leber, P.W. Jones, Polynuclear aromatic hydrocarbons Ann Arbor Science Publishers Inc.,Ann Arbor, MI. ,(1979)