Molecular cloning, expression analyses and functional characterization of a goose-type lysozyme gene from Bostrychus sinensis (family: Eleotridae).

摘要: Abstract In this study, we sequenced and characterized the goose-type lysozyme gene, termed as BsLysG, from Chinese black sleeper (Bostrychus sinensis). The BsLysG encodes 196 amino acids contains a soluble bacterial lytic transglycosylases domain, three catalytic residues (Glu72, Asp85 Asp102) GLMQ motif (Gly97, Leu98, Met99 Gln100). No signal peptide was observed in protein. genomic DNA of five exons four introns. sequence analyses showed that exhibits high similarity with LysG other fishes. Phylogenetic is clustered together its counterparts teleost Real-time PCR found to be ubiquitously expressed ten examined organs sleeper, predominant expression spleen, followed by head kidney peripheral blood. Expression significantly upregulated vivo after either pathogen Vibrio parahemolyticus infection or poly (I:C) challenge blood, kidney, liver spleen organs. purified recombinant (rBsLysG) has optimal activity at 35 °C pH 5.5. rBsLysG exhibited antimicrobial against two Gram-positive bacteria (Micrococcus lysodeikticus Staphylococcus aureus) Gram-negative (Escherichia coli V. parahemolyticus). Scanning electron microscope (SEM) imaging rBsLysG-treated cells displayed morphological deformation. These results indicate involved host immune defense infection.