Helicobacter pylori Induces Increased Expression of the Vitamin D Receptor in Immune Responses

摘要: Background: Vitamin D receptor (VDR) is a member of the nuclear family transcription factors that play critical role in innate immunity. This study examined VDR gastric immune defence against pathogen Helicobacter pylori. Materials and Methods: Seventeen H. pylori-infected patients sixteen controls participated study. The GES-1 cells were transfected with siRNA or incubated without 1a,25(OH)2D3 (100 nmol/L) then infected VDR, cathelicidin antimicrobial protein (CAMP), cytokine mRNA expression levels normal mucosa was determined by qRT-PCR correlated histopathologic degree gastritis. Bactericidal activity measured using colony-forming unit assay. Results: significantly upregulated positively chronic inflammation scores. There significant positive correlation between CAMP pylori-positive mucosa. reduced pylori-induced production conversely increased IL-6 IL8/CXCL8 levels. vitamin agonist activation pylori .1 a,25(OH)2D3 could enhance intracellular killing replicating bacteria, but presence siVDR siCAMP led to decline its bactericidal ability. Conclusions: epithelium up-regulated case infection; thus, plays an important homeostasis host protection from infection.