Curcumin induces human cathelicidin antimicrobial peptide gene expression through a vitamin D receptor-independent pathway

摘要: The vitamin D receptor (VDR) mediates the pleiotropic biologic effects of 1α,25 dihydroxy-vitamin D3. Recent in vitro studies suggested that curcumin and polyunsaturated fatty acids (PUFAs) also bind to VDR with low affinity. As potential ligands for VDR, we hypothesized PUFAs would induce expression known target genes cells. In this study, tested whether these compounds regulated two important — human cathelicidin antimicrobial peptide (CAMP) 1,25-dihydroxyvitamin D3 24-hydroxylase (CYP24A1) monocytic cell line U937, colon cancer HT-29 keratinocyte HaCaT. We demonstrated failed CAMP or CYP24A1 mRNA all three lines, but up-regulated protein levels U937 Curcumin treatment induced promoter activity from a luciferase reporter construct lacking binding site did not increase as determined by chromatin immunoprecipitation assays. These findings indicate induction occurs through receptor-independent manner. conclude do function VDR.