Synergistic induction of human cathelicidin antimicrobial peptide gene expression by vitamin D and stilbenoids

摘要: Scope: The cathelicidin antimicrobial peptide (CAMP) gene is induced by 1α,25-dihydroxyvitamin D 3 (1α,25(OH) 2 ), lithocholic acid, curcumin, nicotinamide, and butyrate. Discovering additional small molecules that regulate its expression will identify new molecular mechanisms involved in CAMP regulation increase understanding of how diet nutrition can improve immune function. Methods results: We discovered two stilbenoids, resveratrol pterostilbene, promoter-luciferase expression. Synergistic activation was observed when either stilbenoid combined with 1α,25(OH) . Both stilbenoids increased mRNA protein levels the monocyte cell line U937 synergy both keratinocyte line, HaCaT. Inhibition targets sirtuin-1, cyclic AMP production c-Jun N-terminal, phosphoinositide AMP-activated kinases did not block induction or Nevertheless, inhibition extracellular signal regulated 1/2 p38 mitogen-activated kinases, combination suggesting these may explain, part, vitamin D. Conclusion: Our findings demonstrate for first time compounds have potential to boost innate response increasing expression, particularly