Differential Requirements of Rab5 and Rab7 for Endocytosis of Influenza and Other Enveloped Viruses

摘要: Enveloped viruses often enter cells via endocytosis; however, specific endocytic trafficking pathway(s) for many have not been determined. Here we demonstrate, through the use of dominant-negative Rab5 and Rab7, that influenza virus (Influenza A/WSN/33 (H1N1) A/X-31 (H3N2)) requires both early late endosomes entry subsequent infection in HeLa cells. Time-course experiments, monitoring viral ribonucleoprotein colocalization with endosomal markers, indicated exhibits a conventional uptake pattern – reaching after approximately 10 min, 40 min. Detection conformation-specific hemagglutinin antibodies did reach fusion-competent form until had trafficked beyond endosomes. We also examined two other enveloped are pH-dependent Semliki Forest vesicular stomatitis virus. In contrast to virus, was inhibited only by expression dominant negative indicating an independence endosome function these viruses. As whole, data provide definitive characterization show differential requirements between