Identification of cis- and trans-acting factors regulating the expression of the human insulin receptor gene.
作者： J.K. Lee , J.W. Tam , M.J. Tsai , S.Y. Tsai
DOI: 10.1016/S0021-9258(18)42881-5
关键词: Hormone response element 、 Binding site 、 Transcription (biology) 、 Gene expression 、 Biology 、 Promoter 、 Transcription factor 、 Deletion Mutagenesis 、 Molecular biology 、 Gene
摘要: The functional organization of the human insulin receptor (hIR) promoter was analyzed by deletion mutagenesis and protein-DNA interaction studies. A series mutants expressed transiently in two hepatocytes, HepG2 PLC. results revealed that region between -692 -345 is essential for efficient transcription hIR gene. Multiple trans-acting factors were identified band shift footprinting analyses. Sp1 binds to a cluster GC boxes GGGAGG hexamers locating at -637 -594. Adjacent boxes, there are regions, from -550 -530 -522 -503, which bind novel factors, IRNF-I IRNF-II. These distributed differentially different cell lines. Linker scanning mutations on GC, GA or binding site significantly decreased transcriptional activity, indicating important activity. In addition, we demonstrated glucocorticoid-dependent induction mRNA vivo conferred glucocorticoid response element promoter. Taken together, these imply gene regulated multiple interactions occurring within defined region.
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