A cluster of four Sp1 binding sites required for efficient expression of the human insulin receptor gene.
作者： E Araki , T Murakami , T Shirotani , F Kanai , Y Shinohara
DOI: 10.1016/S0021-9258(19)67884-1
关键词:
摘要: Fragments of 5'-flanking sequences the human insulin receptor gene were analyzed in transient expression assays after transfection cell lines with an improved low background chloramphenicol acetyl-transferase vector system pSVOOCAT (Araki, E., Shimada, F., Shichiri, M., Mori, and Ebina, Y. (1988) Nucleic Acids Res. 16, 1627). Transfection chimeric acetyltransferase plasmids containing various deletions insertions promoter HIR into CHO COS cells indicated that region between -629 -1 (initiator ATG is +1) sufficient for maximal activity. The DNA element cluster four G-C boxes (-593 to -618) enhanced transcription, examined by vivo. DNase I footprinting gel retardation experiments using partially purified LacZ-Sp1 hybrid proteins showed transcription factor Sp1 can bind promoter. Thus, efficient possibly requires binding transcriptional located -593 -618 base pairs upstream translation initiation codon.
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