Integrin-associated protein/CD47 regulates motile activity in human B-cell lines through CDC42.

摘要: Cell migration requires a dynamic interaction between the cell, its substrate, and cytoskeleton-associated motile apparatus. Integrin-associated protein (IAP)/CD47 is 50-kd cell surface that physically associated with beta3 integrins modulates functions of in various cells. However, B-lymphocytes express beta1 but few integrins, roles IAP/CD47 remain to be determined. Cross-linking by immobilized anti-IAP/CD47 monoclonal antibody (mAb) B6H12, not 2D3, produced signals promote polarization lamellipodia, characteristic morphology during leukocyte migration, pre-B mature B-cell lines (BALL, Nalm6, ONHL-1, Daudi), myeloma (RPMI8226, OPM-2). In presence fibronectin (FN), soluble B6H12 could increase rate activate migratory activity BALL cells FN transwell filter assay. Furthermore, dominant-negative form CDC42 completely blocked B6H12-induced morphologic functional changes without inhibiting phorbol 12-myristate 13-acetate-induced spreading on cells, whereas Rac1 inhibited all these changes. These findings demonstrate B-lymphocytes, may transduce activity, which specifically involved, shows synergistic effect alpha4beta1 migration. would provide new insight into role function.