Anoctamin5 regulates cell migration and invasion in thyroid cancer

摘要: Anoctamin/TMEM16 family members have recently been identified as novel calcium-activated chloride channels, and dysregulation of many participates in tumorigenesis progression. However, the exact role anoctamin5 (ANO5), one member this family, thyroid cancer is still not clarified. In study, we firstly found that expression levels ANO5 was significantly downregulated compared to adjacent normal tissue by mining public GEO database. Subsequently, further demonstrated 69.5% (57/82) clinical tissues using real-time PCR assay. Moreover, western blot assay also showed papillary follicular noncancerous tissues. Furthermore, some biological functional in vitro experiments proved knockdown promotes cell migration invasion but overexpression inhibits these phenotypes. By analyzing gene set enrichment, lower positively associated with JAK/STAT3 signaling pathway. Collectively downregulation affecting