Simultaneous determination of 21 preservatives in cosmetics by ultra performance liquid chromatography.

摘要: Synopsis An ultra performance liquid chromatography (UPLC) method has been developed for the simultaneous determination of 21 preservatives: 2-methyl-4-isothiazoline-3-ketone, bronopol, 5-chloro-2-methyl-4-isothiazoline-3-ketone, benzyl alcohol, 2-phenoxyethanol, methyl-p-hydroxy benzoate, ethyl-p-hydroxy methyl 4-hydroxybenzoic acid iso-propyl ester, propyl-p-hydroxy 4-chloro-3-methylphenol, ethyl 2-phenylphenol, iso-butyl butyl-p-hydroxy 4-chloro-3,5-dimethylphenol, phenyl 2,4-dichloro-3,5-dimethylphenol, 2-benzyl-4-chlorophenol, triclocarban and triclosan in cosmetics. A Waters ACQUITY UPLC BEH C18 column was used with 0.1% formic solution as mobile phase under condition gradient elution. Preservatives were extracted methanol by ultrasonicator, then they analysed UPLC-PDA detector. All these preservatives baseline separated 8.5 min. The pre-treatment samples chromatographic analysis critically examined this study. recoveries ranged from 90.5 to 97.8%, RSD values below 3.2%, all correlation coefficients (r) no less than 0.9997. Thus, could be analysing cosmetic products. Resume Une methode basee sur l’utilisation de l’UPCL (Ultra Performance Liquid Chromatography) a ete developpee pour l’analyse simultanee agents conservateurs dans des produits cosmetiques. Ces sont les 2-methyl-4-isothiazolin-3-one, 5-chloro-2-methyl-4-isothiazolin-3-one, triclocarban, triclosan. Une colonne AQUITY UPCL utilisee avec comme une d’acide formique conditions d’elution. Les ont extraits du aux sons et analyses l’aide d’un detecteur UPLC-PDA. Tous separes en 8,5 mn. La pretraitement echantillons d’analyses chromatographiques examinees facon critique cette etude. deviation standard relative (RSD) est 1.5 ∼ 3.2%. taux recouvrement ∼97.8%, tous ne jamais inferieurs Ainsi, peut-etre analyser cosmetiques.