Bak and Mcl-1 are essential for Temozolomide induced cell death in human glioma.
作者： Catherine Gratas , Quentin Séry , Marion Rabé , Lisa Oliver , François M. Vallette
关键词: Apoptosis 、 RNA interference 、 Programmed cell death 、 Autophagy 、 Bcl-2 family 、 Biology 、 Temozolomide 、 Glioma 、 Cancer research 、 Cell culture
摘要: // Catherine Gratas 1,2,3 , Quentin Sery 1,2,4 Marion Rabe 1,2 Lisa Oliver and Francois M. Vallette 1. Centre de Recherche en Cancerologie Nantes Angers, UMR INSERM 892 / CNRS 6299 2. Universite Nantes, F-44000, France 3. CHU F-44805, France. 4. Institut l’Ouest-Centre Rene Gauducheau, St Herblain Correspondence: Vallette, email: Keywords : temozolomide, glioma, apoptosis Bcl-2 family Received November 29, 2013 Accepted January 1, 2014 Published Abstract Temozolomide (TMZ) is an alkylating agent used for the treatment of glioblastoma multiforme (GBM), main form human brain tumours in adults. It has been reported that TMZ induced DNA lesions subsequently trigger cell death but actual mechanisms involved process are still unclear. We investigated implication major proteins TMZ-induced GBM lines at concentrations closed to reached brain during treatments. did not observe modulation autophagy these we found an induction apoptosis. Using RNA interference, showed that TMZ dependent on pro-apoptotic protein Bak but independent of protein Bax. Apoptosis was enhanced by ABT-737, inhibitor Bcl-2/Bcl-Xl/Bcl-W Mcl-1. The knock-down Mcl-1 expression increased Our results identify a Mcl-1/Bak axis thus unravel target overcome therapeutic resistance toward TMZ.
dissem.in参考文章(40)
Clarissa von Haefen, Bernhard Gillissen, Philipp G Hemmati, Jana Wendt, Dilek Güner, Alicja Mrozek, Claus Belka, Bernd Dörken, Peter T Daniel, Multidomain Bcl-2 homolog Bax but not Bak mediates synergistic induction of apoptosis by TRAIL and 5-FU through the mitochondrial apoptosis pathway Oncogene. ,vol. 23, pp. 8320- 8332 ,(2004) , 10.1038/SJ.ONC.1207971
Roger Stupp, Warren P. Mason, Martin J. van den Bent, Michael Weller, Barbara Fisher, Martin J.B. Taphoorn, Karl Belanger, Alba A. Brandes, Christine Marosi, Ulrich Bogdahn, Jürgen Curschmann, Robert C. Janzer, Samuel K. Ludwin, Thierry Gorlia, Anouk Allgeier, Denis Lacombe, J. Gregory Cairncross, Elizabeth Eisenhauer, René O. Mirimanoff, Radiotherapy plus Concomitant and Adjuvant Temozolomide for Glioblastoma The New England Journal of Medicine. ,vol. 352, pp. 987- 996 ,(2005) , 10.1056/NEJMOA043330
Monika E. Hegi, Annie-Claire Diserens, Thierry Gorlia, Marie-France Hamou, Nicolas de Tribolet, Michael Weller, Johan M. Kros, Johannes A. Hainfellner, Warren Mason, Luigi Mariani, Jacoline E.C. Bromberg, Peter Hau, René O. Mirimanoff, J. Gregory Cairncross, Robert C. Janzer, Roger Stupp, MGMT Gene Silencing and Benefit from Temozolomide in Glioblastoma The New England Journal of Medicine. ,vol. 352, pp. 997- 1003 ,(2005) , 10.1056/NEJMOA043331
Mitsutoshi Nakada, Takuya Furuta, Yutaka Hayashi, Toshinari Minamoto, Jun-ichiro Hamada, The strategy for enhancing temozolomide against malignant glioma. Frontiers in Oncology. ,vol. 2, pp. 98- 98 ,(2012) , 10.3389/FONC.2012.00098
M. W. Pfaffl, A new mathematical model for relative quantification in real-time RT-PCR. Nucleic Acids Research. ,vol. 29, pp. 0- 0 ,(2001) , 10.1093/NAR/29.9.E45
Abbas Fotovati, Rachel Berns, Mary Pambid, Margaret Luk, Richard E. Kast, Esther Kong, Eric Toyota, Stephen Yip, Brian Toyota, Sandra E. Dunn, Joanna Triscott, Cathy Lee, Kaiji Hu, Disulfiram, a drug widely used to control alcoholism, suppresses self-renewal of glioblastoma and overrides resistance to temozolomide Oncotarget. ,vol. 3, pp. 1112- 1123 ,(2012) , 10.18632/ONCOTARGET.604
Richard E Kast, John A Boockvar, Ansgar Brüning, Francesco Cappello, Wen-Wei Chang, Boris Cvek, Q Ping Dou, Alfonso Duenas-Gonzalez, Thomas Efferth, Daniele Focosi, Seyed H Ghaffari, Georg Karpel-Massler, Kirsi Ketola, Alireza Khoshnevisan, Daniel Keizman, Nicolas Magné, Christine Marosi, Kerrie McDonald, Miguel Muñoz, Ameya Paranjpe, Mohammad H Pourgholami, Iacopo Sardi, Avishay Sella, Kalkunte S Srivenugopal, Marco Tuccori, Weiguang Wang, Christian R Wirtz, Marc-Eric Halatsch, None, A conceptually new treatment approach for relapsed glioblastoma: Coordinated undermining of survival paths with nine repurposed drugs (CUSP9) by the International Initiative for Accelerated Improvement of Glioblastoma Care. Oncotarget. ,vol. 4, pp. 502- 530 ,(2013) , 10.18632/ONCOTARGET.969
Tim Mosmann, Rapid colorimetric assay for cellular growth and survival: Application to proliferation and cytotoxicity assays Journal of Immunological Methods. ,vol. 65, pp. 55- 63 ,(1983) , 10.1016/0022-1759(83)90303-4
Bernd Kaina, Kirsten Ochs, Apoptosis Induced by DNA Damage O-Methylguanine Is Bcl-2 and Caspase-9/3 Regulated and Fas/Caspase-8 Independent Cancer Research. ,vol. 60, pp. 5815- 5824 ,(2000)
Kishor K. Bhakat, Dora B. Bocangel, Demetrius M. Kokkinakis, Sydney Finkelstein, Sankar Mitra, S. Clifford Schold, Multifaceted Resistance of Gliomas to Temozolomide Clinical Cancer Research. ,vol. 8, pp. 2725- 2734 ,(2002)