Epi-reevesioside F inhibits Na+/K+-ATPase, causing cytosolic acidification, Bak activation and apoptosis in glioblastoma
作者： Jui-Ling Hsu , Fan-Lun Liu , Lih-Ching Hsu , Hsun-Shuo Chang , Wohn-Jenn Leu
关键词:
摘要: Epi-reevesioside F, a new cardiac glycoside isolated from the root of Reevesia formosana, displayed potent activity against glioblastoma cells. F was more than ouabain with IC50 values 27.3±1.7 vs. 48.7±1.8 nM (P < 0.001) and 45.0±3.4 81.3±4.3 in T98 U87 cells, respectively. However, both were ineffective A172 cell line low Na+/K+-ATPase α3 subunit expression. induced cycle arrest at S G2 phases apoptosis. It also an increase intracellular concentration Na+ but not Ca2+, cleavage exposure N-terminus Bak, loss mitochondrial membrane potential, inhibition Akt induction caspase cascades. Potassium supplements significantly inhibited F-induced effects. Notably, caused cytosolic acidification that highly correlated anti-proliferative activity. In summary, data suggest inhibits Na+/K+-ATPase, leading to overload acidification, Bak activation potential. The PI3-kinase/Akt pathway is caspase-dependent apoptosis ultimately triggered F-treated
impactjournals.com参考文章(55)
Julian Whitaker, Mark F McCarty, Manipulating tumor acidification as a cancer treatment strategy. Alternative medicine review : a journal of clinical therapeutic. ,vol. 15, pp. 264- 272 ,(2010)
Valeria Casavola, Stephan J. Reshkin, Rosa Angela Cardone, Angelo Paradiso, Massimo Tommasino, Antonia Bellizzi, Paclitaxel induces apoptosis via protein kinase A- and p38 mitogen-activated protein-dependent inhibition of the Na+/H+ exchanger (NHE) NHE isoform 1 in human breast cancer cells. Clinical Cancer Research. ,vol. 9, pp. 2366- 2373 ,(2003)
Bin Xu, Xiaobao Jin, Ling Min, Qin Li, Lulu Deng, Hui Wu, Guixian Lin, Lixin Chen, Haifeng Zhang, Chunmei Li, Liwei Wang, Jiayong Zhu, Weizhang Wang, Fujiang Chu, Juan Shen, Hongzhi Li, Jianwen Mao, Chloride channel-3 promotes tumor metastasis by regulating membrane ruffling and is associated with poor survival Oncotarget. ,vol. 6, pp. 2434- 2450 ,(2015) , 10.18632/ONCOTARGET.2966
Jason E. Reynolds, Alan Eastman, Michael A. Barry, Etoposide-induced Apoptosis in Human HL-60 Cells Is Associated with Intracellular Acidification Cancer Research. ,vol. 53, pp. 2349- 2357 ,(1993)
Florian Lang, Else K. Hoffmann, Role of Ion Transport in Control of Apoptotic Cell Death Comprehensive Physiology. ,vol. 2, pp. 2037- 2061 ,(2012) , 10.1002/CPHY.C110046
Pongali B. Raghavendra, Yashin Sreenivasan, Govindarajan T. Ramesh, Sunil K. Manna, Cardiac glycoside induces cell death via FasL by activating calcineurin and NF-AT, but apoptosis initially proceeds through activation of caspases. Apoptosis. ,vol. 12, pp. 307- 318 ,(2007) , 10.1007/S10495-006-0626-3
Catherine Gratas, Quentin Séry, Marion Rabé, Lisa Oliver, François M. Vallette, Bak and Mcl-1 are essential for Temozolomide induced cell death in human glioma. Oncotarget. ,vol. 5, pp. 2428- 2435 ,(2014) , 10.18632/ONCOTARGET.1642
Salvador Harguindey, Stephan J Reshkin, Gorka Orive, Miriam L Wahl, Jose Luis Arranz, Proton Transport Inhibitors as Potentially Selective Anticancer Drugs Anticancer Research. ,vol. 29, pp. 2127- 2136 ,(2009)
Emilie Denicolaï, Nathalie Baeza-Kallee, Aurélie Tchoghandjian, Manon Carré, Carole Colin, Carine Jiguet Jiglaire, Sandy Mercurio, Christophe Beclin, Dominique Figarella-Branger, Proscillaridin A is cytotoxic for glioblastoma cell lines and controls tumor xenograft growth in vivo Oncotarget. ,vol. 5, pp. 10934- 10948 ,(2014) , 10.18632/ONCOTARGET.2541
Marina Cherniavsky-Lev, Ofra Golani, Steven J. D. Karlish, Haim Garty, Ouabain-induced Internalization and Lysosomal Degradation of the Na+/K+-ATPase Journal of Biological Chemistry. ,vol. 289, pp. 1049- 1059 ,(2014) , 10.1074/JBC.M113.517003