Etoposide-induced Apoptosis in Human HL-60 Cells Is Associated with Intracellular Acidification

摘要: Apoptosis is a pathway of cell death characterized by internucleosomal digestion genomic DNA. Such DNA can be induced both physiological stimuli and cytotoxic treatment with many anticancer agents. This has generally been considered to mediated Ca2+/Mg2+-dependent endonuclease that activated increases in intracellular Ca2+. However, we suggest an alternate endonuclease, DNase II, may more likely candidate. In these studies, apoptosis was human HL-60 cells 30-min incubation the topoisomerase II inhibitor etoposide. characteristic began within 3 h removal Morphological indication observed concurrently. Only about 20% underwent at this time; appeared S phase time etoposide treatment. The remainder progressed G2 arrested there for least 48 h. Intracellular Ca2+ pH were measured individual flow cytometry. No changes observed, but acidification up 1 unit occurred 15% correlated course appearance digestion. Cells sorted on basis only acidic showed morphology apoptosis. These results demonstrate involvement apoptotic mechanisms homeostasis as regulators