Electron Cryomicroscopy of Viruses at Near-Atomic Resolutions

作者: Jason T. Kaelber , Corey F. Hryc , Wah Chiu

DOI: 10.1146/ANNUREV-VIROLOGY-101416-041921

关键词: Cell entryResolution (electron density)VirologyGenome packagingVirion assemblyCryo-electron microscopyViral life cycleChemical physicsBiologyAsymmetryTopology (chemistry)

摘要: Recently, dozens of virus structures have been solved to resolutions between 2.5 and 5.0 A by means electron cryomicroscopy. With these we are now firmly within the “atomic age” cryomicroscopy, as studies can reveal atomic details protein nucleic acid topology interactions specific residues. This improvement in resolution has result direct detectors image processing advances. Although enforcing symmetry facilitates reaching near-atomic with fewer particle images, it unfortunately obscures some biologically interesting components a virus. New approaches on relaxing exploring structure dynamics heterogeneity viral assemblies revealed important insights into genome packaging, virion assembly, cell entry, other stages life cycle. In future, novel methods will be required yet-unknown structural conformations viruses, relevant their biological activities. Ultima...

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