作者: Jason T. Kaelber , Corey F. Hryc , Wah Chiu
DOI: 10.1146/ANNUREV-VIROLOGY-101416-041921
关键词: Cell entry 、 Resolution (electron density) 、 Virology 、 Genome packaging 、 Virion assembly 、 Cryo-electron microscopy 、 Viral life cycle 、 Chemical physics 、 Biology 、 Asymmetry 、 Topology (chemistry)
摘要: Recently, dozens of virus structures have been solved to resolutions between 2.5 and 5.0 A by means electron cryomicroscopy. With these we are now firmly within the “atomic age” cryomicroscopy, as studies can reveal atomic details protein nucleic acid topology interactions specific residues. This improvement in resolution has result direct detectors image processing advances. Although enforcing symmetry facilitates reaching near-atomic with fewer particle images, it unfortunately obscures some biologically interesting components a virus. New approaches on relaxing exploring structure dynamics heterogeneity viral assemblies revealed important insights into genome packaging, virion assembly, cell entry, other stages life cycle. In future, novel methods will be required yet-unknown structural conformations viruses, relevant their biological activities. Ultima...