IN VITRO AND IN VIVO HOST RANGE OF ANTICARSIA GEMMATALIS MULTIPLE NUCLEAR POLYHEDROSIS VIRUS
作者： James J. Grasela , Arthur H. McIntosh
DOI: 10.1007/S11626-998-0057-2
关键词:
摘要: A clone of the wild type (wt) Anticarsia gemmatalis multiple nuclear polyhedrosis virus AgMNPV, derived from a geographical isolate (Hondrina, Brazil) and designated AgMNPV-CL4-3A1, was used to determine host range this in six established lepidopteran cell lines: (BCIRL-AG-AM1), Helicoverpa zea (BCIRL-HZ-AM1), Heliothis virescens (BCIRL-HV-AM1), armigera (BCIRL-HA-AM1), Trichoplusia ni (TN-CL1), Bombyx mori (BMN), coleopteran line Anthonomus grandis (BRL-AG-1). In addition, vivo also assayed larvae zea, virescens, ni, homologous species by probit analysis. On basis temporal studies TCID50 values, BCIRL-HV-AM1 cells gave highest extracellular (ECV) titer (9.7×106 TCID50/ml) followed BCIRL-HA-AM1 (8.3×105 BCIRL-AG-AM1 (3.2×105 TCID50/ml). low ECV 1.37×103 TCID50/ml detected TN-CL1 96 h postinoculation, while BRL-AG-1, BMN, BCIRL-HZ-AM1 were nonpermissive AgMNPV-CL4-3A1 on results. AgMNPV had similar restriction profiles that different AcMNPV. The LC50 values 96.9, 564.6, 733.3, 1.1×104 occlusion bodies/cm2 diet for A. gemmatalis, T. respectively.
springer.com
jstor.org 
sci-hub.se 参考文章(22)
Shmuel Razin and David Yogev, E4 – RESTRICTION ENDONUCLEASE ANALYSIS Molecular and Diagnostic Procedures in Mycoplasmology. pp. 355- 360 ,(1995) , 10.1016/B978-012583805-4/50038-8
Richard Shibles, World Soybean Research Conference Iii: Proceedings ,(1985)
Carlo M. Ignoffo, The nuclear-polyhedrosis virus of Heliothis zea (Boddie) and Heliothis virescens (Fabricius): II. Biology and propagation of diet-reared Heliothis Journal of Invertebrate Pathology. ,vol. 7, pp. 217- 226 ,(1965) , 10.1016/0022-2011(65)90041-8
Arthur H. MCINTOSH, Carlo M. IGNOFFO, Characterization of five cell lines established from species of Heliothis. Applied Entomology and Zoology. ,vol. 18, pp. 262- 269 ,(1983) , 10.1303/AEZ.18.262
Arthur H. McIntosh, Carlo M. Ignoffo, Chen Quhou, Mike Pappas, Establishment of a cell line fromHeliothis Armigera (Hbn.) (Lepidoptera: Noctuidae) In Vitro Cellular & Developmental Biology – Plant. ,vol. 19, pp. 589- 590 ,(1983) , 10.1007/BF02619571
A. H. McIntosh, J. J. Grasela, R. L. Matted, Identification of insect cell lines by DNA amplification fingerprinting (DAF) Insect Molecular Biology. ,vol. 5, pp. 187- 195 ,(1996) , 10.1111/J.1365-2583.1996.TB00053.X
G.R. Carner, J.S. Hudson, O.W. Barnett, The infectivity of a nuclear polyhedrosis virus of the velvetbean caterpillar for eight noctuid hosts Journal of Invertebrate Pathology. ,vol. 33, pp. 211- 216 ,(1979) , 10.1016/0022-2011(79)90155-1
James E. Maruniak, Molecular biology of Anticarsia gemmatalis baculovirus Memorias Do Instituto Oswaldo Cruz. ,vol. 84, pp. 107- 111 ,(1989) , 10.1590/S0074-02761989000700020
E.M. Southern, Measurement of DNA length by gel electrophoresis Analytical Biochemistry. ,vol. 100, pp. 319- 323 ,(1979) , 10.1016/0003-2697(79)90235-5
Brad Stiles, I. C. McDonald, J. W. Gerst, T. S. Adams, S. M. Newman, Initiation and characterization of five embryonic cell lines from the cotton boll weevil Anthonomus grandis in a commercial serum-free medium In Vitro Cellular & Developmental Biology – Plant. ,vol. 28, pp. 355- 363 ,(1992) , 10.1007/BF02877059