Molecular characterization of the PR-toxin gene cluster in Penicillium roqueforti and Penicillium chrysogenum: Cross talk of secondary metabolite pathways

摘要: Abstract The PR-toxin is a potent mycotoxin produced by Penicillium roqueforti in moulded grains and grass silages may contaminate blue-veined cheese. derives from the 15 carbon atoms sesquiterpene aristolochene formed synthase (encoded ari 1). We have cloned sequenced four gene cluster that includes 1 P. . Gene silencing of each genes (named prx to 4) resulted reduction 65–75% production indicating encode enzymes involved biosynthesis. Interestingly silenced mutants overproduce large amounts mycophenolic acid, an antitumor compound unrelated pathway suggesting cross-talk acid production. An eleven above mentioned 14-TMS drug/H + antiporter was found genome chrysogenum This has been reported be very poorly expressed transcriptomic study under conditions penicillin (strongly aerated cultures). this apparently silent able produce static culture on hydrated rice medium. Noteworthily, 2.6-fold higher npe10 , strain deleted 56.8 kb amplifiable region containing pen cluster, than parental Wisconsin 54-1255 providing another example between secondary metabolite pathways fungus. A detailed biosynthesis proposed based all available evidence.