Determination of the Labile Iron Pool of Human Lymphocytes using the Fluorescent Probe, CP655
作者： Robert C Hider , Zudong Liu , Yongmin Ma , Frank Petrat
DOI: 10.4137/ACI.S0
关键词:
摘要: The present study introduces a method for determining the labile iron pool (LIP) in human lymphocytes. It is measured using probe CP655, fluorescence of which stoichiometrically quenched by addition iron. intracellular CP655 lymphocytes was increasing concentrations highly lipophilic 8-hydroxyquinoline complex. Intracellular quenching, mediated physiological iron, can be recovered on excess membrane-permeable chelator, CP94. concentration laser scanning microscopy. An ex situ calibration performed “cytosolic” medium based determined and quenching presence LIP healthy to 0.57 ± 0.27 μM. use fluorescent renders it possible record time course uptake chelation CP94 single intact
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