Determination of substituents distribution in carboxymethylpullulans by NMR spectroscopy.
作者： Karine Glinel , Jean Paul Sauvage , Hassan Oulyadi , Jovenka Huguet
DOI: 10.1016/S0008-6215(00)00120-8
关键词:
摘要: The distribution of carboxymethyl substituents in the alpha-(1 --> 6)-linked maltotriosyl repeating units a carboxymethylpullulan (CMP) series was investigated by high resolution NMR spectroscopy on very short oligomers (DPn = 1.2-1.5) obtained acid hydrolysis. A 2D experiments parent pullulan, hydrolysed pullulan and CMP used to assign proton carbon chemical shifts hydrolysates. degree substitution (DS) relative -CH2COONa groups at OH-2, OH-3, OH-4 OH-6 glucose residues (DSi) were determined from H-1 measurements. From set samples, widely different substitution, it observed that C-2 is predominant decreases according order > C-3 C-6 C-4. Taking into account availability each OH group an reactivity hydroxyl defined relation: R-i DSi/n(i), where n(i) number free maltotriose unit (MTU) for given site C-i, found be OH-2 OH-3. (C) 2000 Elsevier Science Ltd. All rights reserved.
elsevier.com
sci-hub.se 参考文章(29)
Frank Alden Bovey, Lynn Jelinski, Chain Structure and Conformation of Macromolecules ,(1983)
Klaus Bock, Christian Pedersen, Carbon-13 Nuclear Magnetic Resonance Spectroscopy of Monosaccharides Advances in Carbohydrate Chemistry and Biochemistry. ,vol. 41, pp. 27- 66 ,(1983) , 10.1016/S0065-2318(08)60055-4
Philip A.J. Gorin, Carbon-13 Nuclear Magnetic Resonance Spectroscopy of Polysaccharides Advances in Carbohydrate Chemistry and Biochemistry Volume 38. ,vol. 38, pp. 13- 104 ,(1981) , 10.1016/S0065-2318(08)60309-1
Werner-Michael Kulicke, Matthias Otto, Andreas Baar, Improved NMR characterization of high-molecular-weight polymers and polyelectrolytes through the use of preliminary ultrasonic degradation Macromolecular Chemistry and Physics. ,vol. 194, pp. 751- 765 ,(1993) , 10.1002/MACP.1993.021940301
D. Bruneel, E. Schacht, Enzymatic Degradation of Pullulan and Pullulan Derivatives Journal of Bioactive and Compatible Polymers. ,vol. 10, pp. 299- 312 ,(1995) , 10.1177/088391159501000402
Stephen J. Angyal, The composition of reducing sugars in solution Advances in Carbohydrate Chemistry and Biochemistry. ,vol. 42, pp. 15- 68 ,(1984) , 10.1016/S0065-2318(08)60122-5
D. Bruneel, E. Schacht, End group modification of pullulan Polymer. ,vol. 36, pp. 169- 172 ,(1995) , 10.1016/0032-3861(95)90688-X
Werner-Michael Kulicke, Arne Henning Kull, Wiebke Kull, Heiko Thielking, Jürgen Engelhardt, Jörn-Bernd Pannek, Characterization of aqueous car☐ymethylcellulose solutions in terms of their molecular structure and its influence on rheological behaviour Polymer. ,vol. 37, pp. 2723- 2731 ,(1996) , 10.1016/0032-3861(96)87634-8
Deane D. McIntyre, Hans J. Vogel, Nuclear Magnetic Resonance Studies of Homopolysaccharides Related to Starch Starch-starke. ,vol. 43, pp. 69- 76 ,(1991) , 10.1002/STAR.19910430209
Hans Bender, Studies of the degradation of pullulan by the decycling maltodextrinase of Flavobacterium sp. Carbohydrate Research. ,vol. 260, pp. 119- 130 ,(1994) , 10.1016/0008-6215(94)80026-X