Transcriptional control of nonfermentative metabolism in the yeast Saccharomyces cerevisiae.

摘要: Although sugars are clearly the preferred carbon sources of yeast Saccharomyces cerevisiae, nonfermentable substrates such as ethanol, glycerol, lactate, acetate or oleate can also be used for generation energy and cellular biomass. Several regulatory networks glucose repression (carbon catabolite repression) involved in coordinate biosynthesis enzymes required utilization substrates. Positively negatively acting complexes pleiotropic proteins have been characterized. The Snf1 (Cat1) protein kinase complex, together with its subunit Snf4 (Cat3) alternative β-subunits Sip1, Sip2 Gal83, plays an outstanding role derepression structural genes which repressed presence a high concentration. One molecular function complex is deactivation by phosphorylation general repressor Mig1. In addition to regulation sugar fermentation, Mig1 influences activators respiration gluconeogenesis, although lesser extent. conversion specific factors into transcriptional activators. This review summarizes cis-acting elements nonfermentative metabolism, corresponding DNA-binding (Hap2-5, Rtg1-3, Cat8, Sip4, Adr1, Oaf1, Pip2), describes interactions among regulators pathway-specific factors. influence source at level, mechanisms post-transcriptional control glucose-regulated stability mRNA discussed briefly.