Characterization of Hex2 protein, a negative regulatory element necessary for glucose repression in yeast.
作者： Dieter NIEDERACHER , Karl-Dieter ENTIAN
DOI: 10.1111/J.1432-1033.1991.TB16187.X
关键词:
摘要: The regulatory HEX2 gene plays an important role in glucose repression the yeast Saccharomyces cerevisiae. hex2 mutants have pleiotropic defects regulation of glucose-repressible enzymes, hexokinase PII synthesis and maltose uptake [Entian, K.-D. & Zimmermann, F.K. (1980) Mol. Gen. Genet. 177, 345-350]. encodes a protein 114137 Da, deduced from its DNA sequence. There were no strong similarities to previously known genes. HEX2-lacZ fusions revealed largely constitutive expression when repressing non-repressing growth conditions compared. Cellular fractionation studies indicated nuclear localization Hex2 protein. mutation was shown be allelic reg1, which releases galactose pathway enzymes [Matsumoto, K., Yoshimatsu, T. Oshima, Y. (1983) J. Bacteriol. 153, 1405-1414]. Overexpression resulted 70% reduction GAL1 under induced conditions. Our support view that is negative element may directly influence transcription, possibly by interaction with transcriptional factors. Deletion experiments identified central core Hex2, spanning only 492 out 1026 amino acid residues, as mainly for repression. are two strongly acidic regions within this part protein, their possible importance discussed.
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