RAD9, RAD17, and RAD24 are required for S phase regulation in Saccharomyces cerevisiae in response to DNA damage

摘要: We have previously shown that a checkpoint dependent on MEC1 and RAD53 slows the rate of S phase progression in Saccharomyces cerevisiae response to alkylation damage. Whereas wild-type cells exhibit slow damage, mec1-1 rad53 mutants replicate rapidly presence or absence DNA In this report, we show other genes (RAD9, RAD17, RAD24) involved damage pathway also play role regulating Furthermore, RAD9, RAD24 fall into two groups with respect both sensitivity regulation phase. demonstrate more dramatic defect is epistatic less severe seen rad9 delta, rad 17 rad24 delta. triple delta rad17 mutant has than mutants. Finally, specificity phenotype by showing repair and/or mgt1 mag1 apn1 rev3 rad18 rad16 dun1-delta 100, sad4-1, tel1 rad26 rad51 rad52-1, rad54 rad14 rad1 pol30-46, pol30-52, mad3 pds1 delta/esp2 pms1 mlh1 msh2 are all proficient at regulation, even though some these mutations confer alkylation.