作者: Milton Chin
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摘要: Successful cryopreservation by the vitrification method depends on high chilling speed. Practitioners of prefer to use liquid nitrogen as cryogen due its inherent safety and low cost. Plunging cryocontainers in a quiescent pool invariably results rate less than theoretical potential. The shortfall is attributed well-known Leidenfrost effect. purpose this invention it provide improve rates during using nitrogen. One feature contacting device that invokes convective heat transfer principles increase In another invention, velocity derived from self-pressurized dewar containing saturated cryogen. self-pressurization achieved ambient heating dewar's contents. embodiment, sub-cooled cryogen, such propane, used tandem with LN2, dewar.