Device and methods for multigradient directional cooling and warming of biological samples

作者: Amir Arav

DOI:

关键词: MechanicsCooling rateFine-tuningSimulationSample (graphics)Freezing pointSeedingProcess (computing)Video imagingTemperature gradientChemistry

摘要: A device for controlled freezing and warming of a biological sample, thawing protocols which the is well suited. The establishes laterally varying thermal gradient provides mechanism moving sample along at rate speed. moved speed that variable cooling or in accordance with appropriate protocol. also allows continuous seeding through process exact point solution. Real time monitoring video imaging enable fine tuning thermodynamic parameters improved control.

参考文章(11)
A. Hubel, E.G. Cravalho, B. Nunner, C. Körber, Survival of directionally solidified B-lymphoblasts under various crystal growth conditions Cryobiology. ,vol. 29, pp. 183- 198 ,(1992) , 10.1016/0011-2240(92)90019-X
H.G. Koebe, A. Werner, V. Lange, F.W. Schildberg, Temperature Gradients in Freezing Chambers of Rate-Controlled Cooling Machines Cryobiology. ,vol. 30, pp. 349- 352 ,(1993) , 10.1006/CRYO.1993.1034
A. Arav, D. Shehu, M. Mattioli, Osmotic and cytotoxic study of vitrification of immature bovine oocytes Reproduction. ,vol. 99, pp. 353- 358 ,(1993) , 10.1530/JRF.0.0990353
W.E. Brower, M.J. Freund, M.D. Baudino, C. Ringwald, An hypothesis for survival of spermatozoa via encapsulation during plane front freezing Cryobiology. ,vol. 18, pp. 277- 291 ,(1981) , 10.1016/0011-2240(81)90099-7
P.S. Fiser, C. Hansen, K.L. Underhill, J.N.B. Shrestha, The effect of induced ice nucleation (seeding) on the post-thaw motility and acrosomal integrity of boar spermatozoa* Animal Reproduction Science. ,vol. 24, pp. 293- 304 ,(1991) , 10.1016/S0378-4320(05)80012-0
A. Arav, B. Rubinsky, E. Seren, J.F. Roche, M.P. Boland, The role of thermal hysteresis proteins during cryopreservation of oocytes and embryos Theriogenology. ,vol. 41, pp. 107- 112 ,(1994) , 10.1016/S0093-691X(05)80055-X
Jeremy Paul Miller, Method and apparatus for freezing. ,(1994)