An hypothesis for survival of spermatozoa via encapsulation during plane front freezing

摘要: Abstract Plane front freezing presents the possibility of encapsulating individual cells in ice phase. The may also be pushed ahead plane interface, as is always case for conventional dendritic freezing, where are thickening dendrite arms. Cells which encapsulated during exposed to hypotonic liquid (pure water) initially upon thawing, while into last freeze hypertonic thawing. Some exposure intercellular prior required build up salt and CPA content intracellular thereby avoid formation at given cooling rate. Encapsulation by a interface should result three regions cell survival sample: an initial region death due formation, final overexposure liquid, intermediate survival, neither damage mechanism has operated lethal level. An advantage over that geometrically separate sample, rather than mixed microstructural level, freezing. Samples containing populations with very high or low rates spermatozoa could obtained simply cutting frozen sample.