Pre-administration of angiopoietin-1 followed by VEGF induces functional and mature vascular formation in a rabbit ischemic model

摘要: Background Angiopoietin-1 (Ang1) and vascular endothelial growth factor (VEGF) play important roles in formation maturation, suggesting that the combination of these two would be a promising therapy for ischemia. However, it remains unclear what best schedule administration cytokines might be. Methods Six experimental groups were used to prepare rabbit ischemic hindlimb model following naked plasmid intramuscular as follows: empty vector (C), single gene (Ang1, A; VEGF, V), Ang-1 followed by VEGF (A − co-administration Ang1 + (V A). Results Thirty days after administration, A V showed significantly increased blood pressure blood-flow recovery limb compared with control group. Histological findings α-smooth muscle-actin (α-SMA) staining revealed had more mature vessels Significantly, highest density α-SMA-positive alone or alone. Angiographic assessment greater arterial diameter Edema, one major adverse effects induced was not found throughout experiments, while severe edema VEGF. Conclusions The pre-administration resulted an improvement hemodynamic status, number covered α-actin-positive mural cells, prevention VEGF-mediated edema. Thus, priming beneficial therapeutic angiogenesis therapy. Copyright © 2003 John Wiley & Sons, Ltd.