Amperometric Detection of DNA by Electroreducation of O2in an Enzyme-Amplified Two-Component Assay

摘要: The two-component type enzyme amplified amperometric DNA assay is described to use an ambient of the substrate labeling enzyme. Although detects only at > 0.5M concentration, a concentration fold higher than sandwich-type assay, it can be run with always available substrate. utilizes screen-printed carbon electrodes (SPEs) which were pre-coated by co-electrodeposited film electron conducting redox hydrogel and 37-base long single-stranded sequence. in hybridizes captures, when present, detection-DNA, labeled bilirubin oxidase (BOD), catalyzing four-electron reduction water. Because electrically connects BOD reaction centers electrode, completion sandwich converts from non-electrocatalytic electrocatalytic for water electrode poised 200 mV vs. Ag/hgCl. advantage or over earlier reported amplifying was horseradish peroxidase, that instead less stable naturally unavailable .