Chimeric DNA/LNA-based biosensor for the rapid detection of African swine fever virus

摘要: African swine fever (ASF) virus is a DNA responsible for severe haemorrhagic in pigs, which (still the absence of vaccination strategies) results high mortality rates. Herein, we present biosensor-based method detection ASF viral blood pigs. The biosensor exploits single-strand probe with locked nucleic acid nucleotides (LNA) substitutions as complementary recognition element conserved region vp72 gene virus. was calibrated using qPCR-quantified extracted from pigs experimentally infected virulent Italian isolate 49/08, genotype I. Globally, proposed showed good sensitivity and specificity, limits (LOD) quantification (LOQ) being 178 245 copies/μL genomic DNA, respectively. reversible nature interaction between DNA/LNA target sequence granted multiple rapid analyses, up to 40 analyses per single surface possible, test requiring approximately 5 min. When applied non-amplified extracts field-infected assay discriminated ASFV-infected ASFV non-infected animals, allowed values falling range 373-1058 DNA. In this range, excellent correlation observed OIE-approved qPCR. This represents promising screening preliminary diagnosis, having major advantages relative rapidity, ease-of-use, reusability sensing surface, low cost test.