Assessment of African Swine Fever Diagnostic Techniques as a Response to the Epidemic Outbreaks in Eastern European Union Countries: How To Improve Surveillance and Control Programs

摘要: This study represents a complete comparative analysis of the most widely used African swine fever (ASF) diagnostic techniques in European Union (EU) using field and experimental samples from animals infected with genotype II ASF virus (ASFV) isolates circulating Europe. To detect ASFV, three different PCRs were evaluated parallel 785 samples. The results showed almost perfect agreement between Universal ProbeLibrary (UPL-PCR) real-time (κ = 0.94 [95% confidence interval {CI}, 0.91 to 0.97]) conventional 0.88 CI, 0.83 0.92]) World Organisation for Animal Health (OIE)-prescribed PCRs. UPL-PCR had greater sensitivity detecting survivors allows earlier detection disease. Compared commercial antigen enzyme-linked immunosorbent assay (ELISA), good-to-moderate 0.67 0.58 0.76]) was obtained, 77.2% test. For antibody detection, five serological methods tested, including ELISAs, OIE-ELISA, confirmatory immunoperoxidase test (IPT). Greater obtained IPT than since able antibodies at an point response, when few are present. exudate tissues dead wild boars that might be useful tool determining whether or not been exposed infection, regardless In conclusion, combination trustworthy method during epidemic outbreaks affecting EU countries 2014. use appropriate tools is critical implementing effective control programs.