Fluorometric quantitation of single-stranded DNA: A method applicable to the technique of alkaline elution
作者： D.L. Stout , F.F. Becker
DOI: 10.1016/0003-2697(82)90177-4
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摘要: Abstract The use of Hoechst dye 33258 for the fluorometric quantitation single-stranded DNA was investigated purpose developing a simple nonradiometric method quantitating in fractions collected during analysis damage by alkaline elution. sensitivity assay allowed amounts as small 100 ng to be quantitated reliably. requirement near-neutral pH necessitated that samples buffered order perform quantitation. However, addition predetermined volume solution each sample is only manipulation required prior fluorescence measurement makes this procedure simplest yet described
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sci-hub.se 参考文章(7)
Daniel L. Stout, Frederick F. Becker, Progressive DNA damage in hepatic nodules during 2-acetylaminofluorene carcinogenesis. Cancer Research. ,vol. 40, pp. 1269- 1273 ,(1980)
John M. Kissane, Eli Robins, The fluorometric measurement of deoxyribonucleic acid in animal tissues with special reference to the central nervous system. Journal of Biological Chemistry. ,vol. 233, pp. 184- 188 ,(1958) , 10.1016/S0021-9258(19)68051-8
Philip C. Hanawalt, Errol C. Friedberg, DNA repair : a laboratory manual of research procedures M. Dekker. ,(1981)
Silvio Parodi, Maurizio Taningher, Leonardi Santi, Marco Cavanna, Luigi Sciaba, Annalisa Maura, Giovanni Brambilla, A practical procedure for testing DNA damage in vivo, proposed for a pre-screening of chemical carcinogens Mutation Research/Environmental Mutagenesis and Related Subjects. ,vol. 54, pp. 39- 46 ,(1978) , 10.1016/0165-1161(78)90133-4
C.F. Cesarone, C. Bolognesi, L. Santi, Improved microfluorometric DNA determination in biological material using 33258 Hoechst Analytical Biochemistry. ,vol. 100, pp. 188- 197 ,(1979) , 10.1016/0003-2697(79)90131-3
Leonard C. Erickson, Rainhardt Osieka, Nancy A. Sharkey, Kurt W. Kohn, Measurement of DNA damage in unlabeled mammalian cells analyzed by alkaline elution and a fluorometric DNA assay. Analytical Biochemistry. ,vol. 106, pp. 169- 174 ,(1980) , 10.1016/0003-2697(80)90133-5
Regina A. Grimek-Ewig, Kurt W. Kohn, Alkaline elution analysis, a new approach to the study of dna single- -strand interruptions in cells. Cancer Research. ,vol. 33, pp. 1849- 1853 ,(1973)