Amnion allografts prepared in the Central Tissue Bank in Warsaw.

摘要: Applications of allogenic amnion grafts range from wound dressing severe burns, dermabrasions and lower extremity ulcer treatments to plastic surgery, laryngology ophthalmology. The aim the present study was elaborate method processing, preservation sterilization human allografts prepared as used mainly for burned patients. During amniotic sac processing (after separation chorion) special attention paid ensure that epithelial side is placed directly on polyester net a support. After application wound, with basement membrane facing outwards; this will promote migration, attachment spreading host cells encouraging epithelialization. Human were preserved by lyophilization or deep-freezing subsequently radiation-sterilized dose 35 kGy. It has been observed, however, lyophilized irradiated are resorbed within few days, while frozen ones better adhere persist even 3 weeks after grafting, therefore, it decided preserve deep-freezing. Since beginning 1998 over 400 (with total surface area 40,000 cm2) have at Central Tissue Bank in Warsaw distributed clinics hospitals throughout country.