Effects of Radiation on the Integrity and Functionality of Amnion and Skin Grafts

摘要: ABSTRACT Amnion and skin grafts represent a very effective treatment of extensive soft tissue defects various origins such as burns, large injuries, granulating wounds chronic wounds. For temporary replacement (grafting) they are used mostly allografts. As in any other kinds allotransplantation, the most important requirements for quality, safety effectivity. Both amnion well differentiated tissues with unique anatomical structures physiologic functions 1 , 2 3 4 5 . Skin is, matter fact, an organ many functions, which integrity functionality organism. When is graft, it can replace just few its original mainly protective barrier ones. The same be applied amnion, although effectiveness enhanced by content different potent healing agents like cytokines growth factors. Anatomically, composed two principle layers – epidermis dermis [1] Epidermis responsible major part function skin. It relatively thin, several cell layers, extracellular matrix. chemical substances cells DNA, RNA, proteins. basal layer attached to basement membrane system representing borderline between dermis. contains special proteins fibronectin, laminin, collagen IV, VII. Compared epidermis, connective fibres matrix, cells. main compounds matrix include collagen, elastin, glycosaminoglycans (mostly hyaluronic acid heparan sulphate). principal mechanical strength, carrier blood vessels, nerves, appendages. thickness variable depends on region. thinnest area upper eyelids genitalia (0.5 mm), thickest back (2 mm). Amnion, compared skin, much thinner (0.05-0.2 mm) five layers. single cuboid membrane; three compact layer, fibroblastic spongy layer. does not contain lymphatic or nervous tissue. embryonic origin. Radiation sterilisation increase biological order prevent transmission microorganisms causing diseases from donor recipient 6 7 8 9 irradiation doses sterilise do merely devitalise tissue, but also cause structural changes components grafts. Structures containing molecules some proteins, acid, vulnerable irradiation. issue that these will adversely affect extent integrity, adherence grafts, properties determinants substantial substitute functions.