Misincorporation signatures for detecting modifications in mRNA: Not as simple as it sounds.
作者： Aldema Sas-Chen , Schraga Schwartz
DOI: 10.1016/J.YMETH.2018.10.011
关键词:
摘要: Abstract Post-transcriptional modification on mRNA has become a field of intense interest in recent years, and next-generation sequencing based technologies are constantly emerging to detect an increasing number modifications at transcriptome-wide level. Some these approaches identification misincorporation events induced by reverse transcriptase modified sites. Although conceptually trivial, sensitive specific such is challenge prone surprising artifacts, which can lead substantially inflated estimates the abundance diverse modifications. Here we discuss sources some artifacts delineate overcome them.
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