Delivering Growth Factors through a Polymeric Scaffold to Cell Cultures Containing both Nucleus Pulposus and Annulus Fibrosus.

摘要: Aim To design a novel, polyvinyl alcohol (PVA)-based polymeric scaffold that permits the controlled release of insulin-like growth factor 1 (IGF-1)/bone morphogenetic protein (BMP)-2 following intervertebral disc administration. Material and methods The drug delivery system was composed two different solutions formed within seconds coming into contact with each other. Swelling, pH, temperature tests analysis factors (GFs) from this were performed. kinetics GFs determined through enzyme-linked immunosorbent assay (ELISA). Cell proliferation viability monitored microscopy analyzed using an MTT acridine orange/propidium iodide (AO/PI) staining. Chondroadherin (CHAD), hypoxia inducible factor-1 alpha (HIF-1?), collagen type II (COL2A1) gene expressions quantitative real-time polymerase chain reaction (qRT-PCR) to show effects IGF-1/BMP-2 administration on annulus fibrosus cell (AFC)/nucleus pulposus (NPC) cultures. For statistical evaluation obtained data, experimental groups compared post hoc Tukey's test variance. Results allowed for IGF-1 BMP-2 in time intervals. It observed as application increased, number cells degree extracellular matrix development increased AFC/NPC AO/PI staining showed retained their specific morphology continued proliferate. HIF-1? CHAD expression time-dependent manner, no COL2A1 AFC/ NPC cultures observed. Conclusion designed may be used alternative method after further vivo studies. Such prototype scaffolds innovative technology targeted therapies reconstructive neurosurgical interventions.