Tagging and mapping of a rice gall midge resistance gene, Gm8, and development of SCARs for use in marker-aided selection and gene pyramiding.

摘要: Using amplified fragment length polymorphisms (AFLPs) and random polymorphic DNAs (RAPDs), we have tagged mapped Gm8, a gene conferring resistance to the rice gall midge (Orseolia oryzae), major insect pest of rice, onto chromosome 8. AFLPs, two fragments, AR257 AS168, were identified that linked resistant susceptible phenotypes, respectively. Another phenotype-specific marker, AP19587, was also using RAPDs. SCAR primers based on sequence fragments AS168 failed reveal polymorphism between parents. However, PCR regions flanking revealed phenotype-specific. In contrast, carried out phenotype-associated produced monomorphic fragment. Restriction digestion these Nucleotide BLAST searches three show strong homology PAC BAC clones formed contig representing short arm amplification above-mentioned larger population, derived from cross indica varieties, Jhitpiti (resistant parent) TN1 (susceptible parent), showed there is tight linkage markers Gm8 locus. These markers, therefore, potential for use in marker-aided selection pyramiding along with other previously genes [Gm2, Gm4(t), Gm7].