BAC-derived markers for assaying the stem rust resistance gene, Sr2, in wheat breeding programs
作者： MD McNeil , R Kota , Etienne Paux , D Dunn , R McLean
DOI: 10.1007/S11032-007-9152-4
关键词:
摘要: Durable broad-spectrum, adult-plant stem rust resistance in wheat conferred by the Sr2 gene has remained effective against Pucciniagraminis f. sp tritici worldwide for more than 50 years. The been positioned on physical map of to distal 25% portion short arm chromosome 3B. Selection this breeding programs within Australia performed so far through use linked pseudo black chaff (PBC) phenotype and microsatellite markers Xgwm389 Xgwm533 that flank gene. molecular a genetic interval approximately 4 cM equating distance over 10 Mbp. Recently, 3B-specific BAC library was developed established region. Analysis sequence minimal tiling path-BAC clones region containing enabled development three new were mapped Xgwm389–Xgwm533 tightly Screening wide range germplasm with these demonstrated their usefulness marker-assisted selection Australian programs.
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