Transgenic DNA integrated into the oat genome is frequently interspersed by host DNA.

摘要: Integration of transgenic DNA into the plant genome was investigated in 13 oat (Avena sativa L.) lines produced using microprojectile bombardment with one or two cotransformed plasmids. In all transformation events, integrated consisted intact transgene copies that were accompanied by multiple, rearranged, and/or truncated fragments. All fragments cosegregated, indicating they at single gene loci. Analysis structure loci indicated interspersed host genomic DNA. The number insertions within varied from 2 to 12 among lines. Restriction endonucleases do not cleave introduced plasmids restriction ranging 3.6 about 60 kb length hybridizing a probe comprising Although size interspersing locus is unknown, sizes transgene-hybridizing entire must be least 35–280 kb. observation analyzed exhibited interspersion multiple clustered transgenes suggests predominating integration mechanism. We propose replication forks could account for observed