Segregation of transgenes in maize.
作者： T. Michael Spencer , James V. O'Brien , William G. Start , Thomas R. Adams , William J. Gordon-Kamm
DOI: 10.1007/BF00034949
关键词:
摘要: Progeny recovered from backcrossed transgenic maize tissue culture regenerants (R0) were analyzed to determine the segregation, expression, and stability of introduced genes. Transgenic A188×B73 R0 plants (regenerated embryogenic suspension cells transformed by microprojectile bombardment; see [9]) pollinated with nontransformed B73 pollen. Inheritance a selectable marker gene, bar, nonselectable uidA, was in progeny (R1) representing four independent transformation events. Activity bar gene product, phosphinothricin acetyltransferase (PAT), assessed comprising R1 populations. The number containing PAT activity per total for each population 2/7, 19/34, 3/14 73/73. Molecular analysis confirmed segregation three populations lack one population. Cosegregation indicated genetic linkage uidA all Analysis numerous R2 derived crossing inbreds revealed 1:1 lines, including line that failed segregate generation. Integrated copies appeared be unstable or poorly transmitted.
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