Transgenic Maize by Electroporation of Pectolyase-Treated Suspension Culture Cells

摘要: Recent progress in the development of direct gene transfer techniques has made genetic manipulation important cereal crops possible. Direct DNA-delivery to protoplasts first yielded transgenic rice (Shimamoto et al., 1989) and ultimately maize (Omirulleh 1993). Given difficulties developing protoplastable cell lines problems associated with regenerating plants from (Potrykus, 1990), other strategies have been pursued. Microprojectile bombardment (Klein 1987) was technique successfully employed for transformation (Gordon-Kamm 1990; Fromm Walters al, 1992) wheat (Vasil 1992). Alternatively, Dekeyser al. (1990) demonstrated that it possible introduce DNA into intact monocot tissues using electroporation. Transient expression reporter genes leaf bases rice, wheat, maize, barley. Subsequently, D’Halliun (1992) used electroporation combination enzymatic or mechanical wounding, transform immature embryos Type I callus, giving rise plants. Here we describe a procedure suspension culture cells by present analysis