Transcriptome Profile of the Response of Paracoccidioides spp. to a Camphene Thiosemicarbazide Derivative
作者： Lívia do Carmo Silva , Diana Patrícia Tamayo Ossa , Symone Vitoriano da Conceição Castro , Ludmila Bringel Pires , Cecília Maria Alves de Oliveira
DOI: 10.1371/JOURNAL.PONE.0130703
关键词:
摘要: Paracoccidioidomycosis (PCM) is a systemic granulomatous human mycosis caused by fungi of the genus Paracoccidioides, which geographically restricted to Latin America. Inhalation spores, infectious particles fungus, common route infection. The PCM treatment choice azoles such as itraconazole, but sulfonamides and amphotericin B are used in some cases despite their toxicity mammalian cells. current availability treatments highlights need identify characterize novel targets for antifungal well search new compounds obtained from natural sources or chemical synthesis. To this end, we evaluated activity camphene thiosemicarbazide derivative (TSC-C) compound on Paracoccidioides yeast. determine response spp. TSC-C, analyzed transcriptional profile fungus after 8 h contact with compound. results demonstrate that lutzii induced expression genes related metabolism; cell cycle DNA processing; biogenesis cellular components; transduction/signal; rescue, defense virulence; transport, transport facilities routes; energy; protein synthesis; fate; transcription; other proteins without classification. Additionally, observed intensely inhibited Analysis fluorescence microscopy flow cytometry revealed production reactive oxygen species. Using an isolate down-regulated SOD1 gene (SOD1-aRNA), sought function yeast cells against Mutant were more susceptible demonstrating importance presented herein suggest TSC-C promising candidate treatment.
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