Image Analysis by Electron Microscopy of Two-Dimensional Crystals Developed on a Mercury Surface of Chaperonin from Thermus thermophilus

摘要: Two-dimensional crystals of functional chaperonin molecules, which are protein complexes cpn60 and cpn10, isolated from Thermus thermophilus were prepared on a mercury surface under oxygen atmosphere observed by electron microscope after transferring them to carbon coated specimen grids. The showed the hexagonal lattice with unit cell dimensions = b 12.4 nm gamma 120 degrees. averaged image at 3 resolution has doughnut-like shape seven peripheral masses central cavity. Preincubation MgATP changed mobility in non-denaturating PAGE but did not cause distinguishable change shape. location cpn10 molecule is discussed.