PROTEIN CONFORMATIONAL CHANGES IN THE μs TIME REGION INVESTIGATED WITH A LASER PULSE PHOTOLYSIS TECHNIQUE

摘要: Publisher Summary This chapter describes two experimental techniques. One is used to determine the rate of photolysis and quantum yield photolabile precursors. These measurements are important because properties photolytic reaction (rate, yield) depend on pH, buffer concentration, substituents α-carbon protecting group, leaving group. The second technique measure at which closed-channel form converted open-channel protein. compounds in 0-nitrobenzyl group utilized can be measured by studying a UV photolysis-induced transient chromophore with an absorbance maximum between 400 440 nm, aci-nitro intermediate. Using apparatus methods, formation open transmembrane channels investigated, hence transformation protein conformations that give rise them and, therefore, obtain information about rates signals transmitted cells nervous system received, stored transmitted. techniques not only acetylcholine receptor cultured but also other receptors from specific areas system.