Variation in levels of cytochrome P4501A, 2B, 2E, 3A and 4A-immunopositive proteins in digestive gland of indigenous and transplanted mussel Mytilus galloprovincialis in Venice Lagoon, Italy

摘要: Mytilus galloprovincialis digestive gland microsomes were prepared from (i) indigenous populations sampled a clean reference (Lido) and an urban-contaminated site (Salute) (ii) mussels transplanted for up to 3 weeks Lido industrial-contaminated (CVE) in Venice Lagoon, Italy. Western blot analysis was performed using antibodies five mammalian or fish CYP forms (1A, 2B, 2E, 3A, 4A). Simultaneously run M. edulis partially purified aided identification of immunopositive bands. Levels CYP1A, CYP2E, CYP4A-immunopositive proteins 50 300% higher Salute compared (p < 0.05). Three after transplantation CVE, levels only the CYP1A-immunopositive protein determined be (63%) than 0.05), indicating that anti-CYP1A shows greater specificity contaminant-inducible form other antibodies.