Assessment of the impact of organic pollutants on goby (Zosterisessor ophiocephalus) and mussel (Mytilus galloprovincialis) from the Venice Lagoon, Italy: Biochemical studies

摘要: The use of cytochrome P4501A (CYP1A) and other measurements as biomarkers was investigated in liver goby (Z. ophiocephalus) digestive gland mussel (M. galloprovincialis) from several sites the Venice lagoon part UNESCO-MURST Lagoon Ecosystem Project. Most tissue contaminants (PAHs, PCBs, DDTs) biochemical varied seasonally. Elevated 7-ethoxyresorufin O-deethylase activity CYP1A-protein levels were correlated with high contaminant at industrial Porto Marghera site. On occasions, activities antioxidant enzymes catalase putative DT-diaphorase (resorufin reductase activity) male but not female also higher than sites, no differences seen Superoxide dismutase (SOD) activity. A range (SOD, catalase, NADPH-cytochrome c glutathione S-transferase activities, P450 ‘418-peak’ contents) showed little difference between sites. However, indications obtained elevated CYP1A1-like mRNA, CYP1A-like protein metabolism benzo[a]pyrene to free metabolites mussels compared a site Adriatic Sea. studies demonstrate usefulness CYP1A biomarker for organic pollution fish indicate some potential its application molluscs.