Purification and assays of Saccharomyces cerevisiae homologous recombination proteins.
作者： Stephen Van Komen , Margaret Macris , Michael G. Sehorn , Patrick Sung
DOI: 10.1016/S0076-6879(06)08028-1
关键词:
摘要: Homologous recombination is an important means of eliminating DNA double strand breaks from chromosomes. The homologous reaction mediated by the Rad51 recombinase, which requires a number ancillary factors for maximal efficiency. development purification procedures and biochemical assays yeast other proteins has allowed investigators to begin dissecting hierarchy physical functional interactions among these protein that govern integrity machinery. studies done with have helped formulate conceptual frameworks guide similar endeavors in eukaryotes, including humans. Continuing efforts reconstituted systems comprise will undoubtedly continue provide insights into mechanistic intricacy
sci-hub.se 参考文章(41)
Fred Sherman, Getting started with yeast. Methods in Enzymology. ,vol. 350, pp. 3- 41 ,(2002) , 10.1016/S0076-6879(02)50954-X
Akira Shinohara, Tomoko Ogawa, Stimulation by Rad52 of yeast Rad51- mediated recombination Nature. ,vol. 391, pp. 404- 407 ,(1998) , 10.1038/34943
James H. New, Tomohiko Sugiyama, Elena Zaitseva, Stephen C. Kowalczykowski, Rad52 protein stimulates DNA strand exchange by Rad51 and replication protein A Nature. ,vol. 391, pp. 407- 410 ,(1998) , 10.1038/34950
L.A. Henricksen, C.B. Umbricht, M.S. Wold, Recombinant replication protein A: expression, complex formation, and functional characterization. Journal of Biological Chemistry. ,vol. 269, pp. 11121- 11132 ,(1994) , 10.1016/S0021-9258(19)78100-9
Galina Petukhova, Sabrina Stratton, Patrick Sung, Catalysis of homologous DNA pairing by yeast Rad51 and Rad54 proteins Nature. ,vol. 393, pp. 91- 94 ,(1998) , 10.1038/30037
Galina Petukhova, Stephen Van Komen, Sefton Vergano, Hannah Klein, Patrick Sung, Yeast Rad54 Promotes Rad51-dependent Homologous DNA Pairing via ATP Hydrolysis-driven Change in DNA Double Helix Conformation Journal of Biological Chemistry. ,vol. 274, pp. 29453- 29462 ,(1999) , 10.1074/JBC.274.41.29453
Patrick Sung, Lumir Krejci, Stephen Van Komen, Michael G. Sehorn, Rad51 Recombinase and Recombination Mediators Journal of Biological Chemistry. ,vol. 278, pp. 42729- 42732 ,(2003) , 10.1074/JBC.R300027200
H Ito, Y Fukuda, K Murata, A Kimura, Transformation of intact yeast cells treated with alkali cations. Journal of Bacteriology. ,vol. 153, pp. 163- 168 ,(1983) , 10.1128/JB.153.1.163-168.1983
Jonathan A. Eisen, Kevin S. Sweder, Philip C. Hanawalt, Evolution of the SNF2 family of proteins: subfamilies with distinct sequences and functions Nucleic Acids Research. ,vol. 23, pp. 2715- 2723 ,(1995) , 10.1093/NAR/23.14.2715
Steven J. Brill, Bruce Stillman, Yeast replication factor-A functions in the unwinding of the SV40 origin of DNA replication Nature. ,vol. 342, pp. 92- 95 ,(1989) , 10.1038/342092A0