Atomic Force Microscopy of Chromatin

摘要: Atomic force microscopy or scanning tunneling (AFM/STM) is a powerful single molecule tool for the visualization of biological materials at sub-nanometer resolution. AFM versatile because it can directly measure physical properties due to its sensitivity picoNewton scales, thus enabling dissection molecular forces. STM/AFM has been considered revolutionary since discovery 32 years ago, and mere 7 after invention, Binnig Rohrer were awarded Nobel Prize in Physics, along with Ruska, inventor first electron microscope. In subsequent years, evolved fast, range applications expanding encompass all sciences. Despite it’s popularity physics, engineering, material chemistry sciences, remained less prominent biologists, who are much more familiar techniques like (EM), X-ray crystallography nuclear magnetic resonance (NMR). The relatively low cost microscope, ease sample preparation, lack any requirement staining, freezing, excessive denaturation preservation, should make an ideal laboratories interested imaging manipulating samples down macromolecules nanoscale. this chapter, we will discuss how be effective high-resolution technique structural interaction studies biology.