Consensus interferon induces peak mRNA accumulation at lower concentrations than interferon-α2a
作者： S.B. KLEIN , L.M. BLATT , M.W. TAYLOR
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摘要: ABSTRACT The biological activity of a novel recombinant interferon, r-metIFN-con1, which represents consensus sequence the most commonly appearing amino acids at each locus 14 naturally occurring IFN-αs, was assessed and compared to that IFN-α2a. increase in cellular mRNA levels for three IFN-inducible genes served as quantitative measure effectiveness stimulation by IFNs. Three cell lines were treated with equimolar amounts two IFNs encompassing 5 log range extracted five different times after treatment. In all cases, r-metIFN-con1 produced increases lower concentrations than HLA-DRα mRNA, is not affected IFN-α ME180 or Dandi cells, also r-metIFN-con1. However, Eskol both effected an similar levels. effective approximately 10-fold molar concentrations. At (10-fold dose r-metIFN-con...
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