Activation of the human beta-interferon gene requires an interferon-inducible factor.

摘要: beta-Interferon (beta-IFN) gene expression can be induced by poly(I)-poly(C) or virus, but there is considerable variation in the extent of induction between different cell lines. We characterized two poorly inducible human lines, HeLa and 143 thymidine kinase negative (143 tk-), to define cellular factors involved activation beta-IFN gene. show that deficiency these cells complemented fusion highly mouse cells. conclude are deficient a trans-acting factor required for B-IFN activation. The level tk- also increased priming with IFN before induction. If carried out presence cycloheximide, approximately 200-fold increase observed. requires an IFN-inducible only expressed at low levels unprimed