Characterization of the murine macrophage mannose receptor: demonstration that the downregulation of receptor expression mediated by interferon-gamma occurs at the level of transcription.

作者: N Harris , M Super , M Rits , G Chang , RA Ezekowitz

DOI: 10.1182/BLOOD.V80.9.2363.2363

关键词:

摘要: The macrophage mannose receptor (MMR) is a 175-Kd cell-surface transmembrane glycoprotein that expressed on tissue macrophages where it functions both to mediate the uptake of mannose-rich glycoproteins and as phagocytic for bacteria, yeasts, other pathogenic microorganisms. In this report we describe cloning full-length cDNA mouse investigate level at which interferon gamma (IFN-gamma) downregulates expression. latter marker functional state cell high levels are resident inflammatory macrophages, whereas cells activated by treatment with IFN-gamma have decreased-to-absent murine MMR contains an open reading frame predicts protein 1,456 amino acids. Transient expression in heterologous shows encodes receptor. deduced acid sequence has overall 82% homology human such, ectodomain N-terminus cysteine-rich followed fibronectin type II domain eight carbohydrate recognition domains (CRDs). linked hydrophobic region 46-amino cytoplasmic tail. All CRDs particularly well conserved, especially CRD4, 92% equivalent protein. Steady-state mRNA were measured line J774E, known express surface. These decreased 4- 8-hour incubation IFN-gamma, but almost abolished overnight cytokine. Nuclear run-on experiments showed inhibits gene transcription. Therefore, regulation provides novel system study mechanisms cytokine represses

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