Statistical media optimization for enhanced production of fibrinolytic enzyme from newly isolated Proteus penneri SP-20

摘要: Abstract Thrombolytic enzymes are invaluable for combating cardiovascular diseases. In this study a thorough isolation resulted, potent fibrinolytic enzyme producer which was identified as Proteus penneri SP-20 using 16S rRNA sequencing. It secretes extracellular protease possesing activity confirmed through the analysis of fibrin degradation by SDS-PAGE, phase contrast microscopy and zymography. Response surface methodology is used media optimization newly isolated P. SP-20. Plackett Burman (PB) design employed selection critical constituents while; further carried out Central composite rotatable (CCRD). Casein, yeast extract, maltose MnCl2·4H2O were medium components selected on basis PB design. Optimized obtained CCRD casein; 12.58 g l−1, extract; 17.27 g l−1, maltose; 1.65 g l−1, MnCl2·4H2O; 0.564 g l−1. As compared with initial 38.19 Units yielded 796.82 Unit activity, increasing overall production 20.86 fold. Hence model reliable process optimization.